WebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions … WebIn addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression …
GCaMP - Wikipedia
WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. WebDec 16, 2024 · CRITICAL: For calcium imaging experiments, a mix of both viral constructions (AAV2.5-gfaABC1D-cyto-GCaMP6f (viral titer range 2–6 × 10 13 vg/ml) and AVV2.5-GFAP-Cre-mCherry (4.3 × 10 12 vg/ml; 1:1 ratio) are injected. c. Place the tip of the needle in the first position and make a mark scratching softly on the surface of the skull … cylanceprotect free download
Opto-electrical bimodal recording of neural activity in awake head ...
GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase. When bound to Ca , GCaMP fluoresces green with a peak excitation wavelength of 480 nm and a peak emission wavelength of 510 nm. It is used in biological … WebNov 10, 2024 · In this study, we expressed highly sensitive calcium indicators, GCaMP6f [ 9] and R-CaMP2 [ 10] in astrocytes and neurons, respectively, to observe simultaneously the astrocytic and neuronal visual responses in the V1 of awake mice. We found that neuromodulator-mediated global activities dominated in the astrocytic activities. WebJul 11, 2024 · AAV2/5-gfaABC1D-cyto-GCaMP6f (Addgene 52925); viral titer 6.13 × 10 13. 5. Stereotaxic apparatus . 6. Microinjector. 7. Needle/glass micropipettes. 8. Isoflurane. 9. Scalpels. 10. Surgical drill. 11. Heat blanket. 12. Mouse body temperature probe. 2.2 Optical Fiber Implantations 1. cylanceprotect latest release