WebCTS Dynabeads CD3/CD28 preferentially isolate CD3 + CD28+ cells. PBMCs were incubated for 30 min with CTS Dynabeads CD3/CD28 PBMCs were incubated for 30 min with CTS Dynabeads CD3/CD28 at a ratio of 3:1 beads:cells, and the negative (nonisolated) fraction was analyzed and used to calculate isolation efficiency. WebCTS ™ Dynabeads CD3/CD28 contains 4 × 108 beads/mL in phosphate buffered saline (PBS), pH 7.4, with 0.1% recombinant human serum albumin (recombinant HSA). …
Dynabeads Human T-Activator CD3/CD28/CD137 - Thermo …
WebFigure 2. Strong isolation efficiency with no impact on cell viability. Using CTS Dynabeads CD3/CD28 and the CTS DynaCellect Magnetic Separation System, (A) isolation efficiency was greater than ~86% (N=4) while (B) cell viability was maintained at >87%, as determined by flow cytometry. Figure 3. Activation of isolated target cells is confirmed. On WebJan 16, 2015 · Dynabeads CD3/CD28 Cell Therapy System (CTS)-activated or antigen-specific T cells expanded using the xeno-free SR, CTS Immune Cell SR, showed comparable growth kinetics observed with cell culture media supplemented with HS or FBS. Importantly the xeno-free SR supplemented medium supported the optimal expansion of … simple fiber customer service number
A simple and effective method to purify and activate T cells for ...
WebNov 19, 2024 · CTS™Dynabeads™CD3/CD28 requires no upstream T cell selection as this technology simultaneously isolates and activates naïve and early memory T cells based on CD3 and CD28 co-expression. WebFollowing isolation and activation using the CTS DynaCellect system and CTS Dynabeads CD3/CD28 magnetic beads, the monocyte levels were reduced to 0.1%, enabling the customer to achieve 92.9% cell purity during the third and final run (Figure 2). Figure 1. T cells are isolated with robust efficiency obtained with WebDynabeads® Human T-Activator CD3/CD28 are uniform 4.5-μm, superparamagnetic polymer beads coated with an optimized mixture of monoclonal antibodies against the CD3, CD28, and CD137 cell surface molecules of human T cells. The CD3 antibody is specific for the epsilon chain of human CD3, which is considered to be a subunit of the TCR complex. simplefiber communications