WebH2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for DNA repair and maintaining genomic stability and for … WebFeb 3, 2024 · For both cytospin and tissue slide staining of γH2AX, a biotinylated γ-H2AX monoclonal antibody was used (clone JBW301 from Millipore). Staining procedure and quantification method were previously …
Histone H2AX phosphorylation: a marker for DNA damage
WebNov 18, 2024 · Squamous esophageal carcinoma is a common pathological type of esophageal carcinoma around the world. The prognosis of esophageal carcinoma is usually poor and diagnosed at late stages. Recently, research suggested that genomic instability occurred in esophageal cells during the development of esophageal squamous cell … WebOct 1, 2024 · Example of Analysis of PAR and γ-H2A.X Staining (A to D) Raw images of immunofluorescence staining for PAR (red) and wheat germ agglutinin (WGA) (green) with 4,6-diamidino-2-phenylindole (DAPI) (blue) nuclear counterstain using an endomyocardial biopsy specimen (white bar = 50 μm) from LVRR-negative and LVRR-positive patients, … lamura
Ji-Hun Shin - Postdoctoral Researcher - UC Irvine LinkedIn
WebApr 28, 2016 · Fluorescent images of DAPI (blue), H2A (yellow) and H4(red) staining. (A) There was positive staining for H2A and H4 in decondensed nuclei (a–c,g,h) but little or no staining in decondensed cauda epididymis Sp nuclei (i–l,o,p). No signals were observed in the control group (d). Both 10-nm (H2A) and 20-nm (H4) gold particles were observed in ... WebFeb 22, 2024 · The phosphorylation of histone H3 at serine 10 (p-H3S10) has been shown to be closely correlated with mitotic chromosome condensation. We previously reported that intracellular silver nanoparticles (AgNPs) release Ag ions that alter actin filament dynamics, leading to the activation of Aurora kinases and the formation of p-H3S10 through a … WebJan 31, 2024 · The nonessential amino acid asparagine can only be synthesized de novo by the enzymatic activity of asparagine synthetase (ASNS). While ASNS and asparagine have been implicated in the response to numerous metabolic stressors in cultured cells, the in vivo relevance of this enzyme in stress-related pathways remains unexplored. lamura black sirius