WebHuntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, … B1.21 cells17are based on HEK293 cells and express full-length wild-type 17QHTT upon induction with doxycycline. The expression plasmid pBSK/2-CMV-HAP40-TS was constructed to express the human HAP40 protein (NCBI RefSeq NP_036283.2) with a C-terminal Twin-Strep-tag under the control of the hCMV … Meer weergeven The following antibodies were used: anti-Flag M2 (Sigma), anti-HAP40 (Santa Cruz SC-69489), anti-Strep (IBA 2-1507-001) and anti-HTT … Meer weergeven HEK293-based C2.6 cells17 (2 × 108) expressing Flag-tagged full-length polyglutamine-expanded 46QHTT at low levels were … Meer weergeven Sucrose gradients (5–20%) in 25 mM HEPES, 300 mM NaCl, 0.1% CHAPS, pH 8.0 were generated by an automatic gradient maker (Gradient Master, Biocomp Instruments). A volume of 120 μl of Flag-tag-purified … Meer weergeven The purification of HTT alone has been described17. For purification of the HTT–HAP40 complex, 2 × 108 B1.21-HAP40TS cells were collected 72 h after induction with … Meer weergeven
Comparative molecular dynamics simulations of pathogenic and …
Web15 jul. 2014 · Together, our findings reveal that Htt is necessary for mitochondrial structure and function from the earliest stages of embryogenesis, providing a molecular … Web18 mei 2010 · Huntingtin Early endosome Note: The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington disease neurons. Shuttles … recipe for beef shank stew
3064 - Gene ResultHTT huntingtin [ (human)] - National Center for ...
Web10 apr. 2024 · Polyglutamine expansion at the N-terminus of the huntingtin protein exon 1 (Htt-ex1) is closely associated with a number of neurodegenerative diseases, which … Web15 nov. 2005 · Although the structure of huntingtin remains unknown, a lot of information is now available about its function in brain cells and the interactions of its 3,144 amino … Web13 okt. 2011 · Huntington's disease is a neurodegenerative disorder caused by a polyglutamine (polyQ) expansion in the N-terminal fragment of the Huntingtin (Htt) … recipe for beef seasoning